Development of Fast, Precise and Selective RP-HPLC Methods for Identification of Possible Degradation Products of Ivermectin in Isolated Rat Hepatocytes and in Different pH Media

Abstract: Aim: A new stability indicating RP-HPLC based methods were developed to identify the possible degradation products of Ivermectin in isolated rat hepatocytes and different pH media using a C18 RP column. Complete validation, including linearity, accuracy, recovery, precision, robustness, stability, peak purity, was performed. Materials Methods: HPLC system UltiMateDionex 3000 DAD with LiChrosorb Column used this study. During investigation, for removal interfering fraction purification procedure, consisting protein precipitation followed by double filtration developed. Results: Method I applied effectively results indicated two major hepatic metabolites Ivermectin. II monitor chemical close physiological conditions. The appearance proportional decrease sample concentration indicate liability buffer pH12. Conclusion: obtained demonstrated that cells alkali are different. Key words: Ivermectin, In vitro, Metabolism, Chemical RP-HPLC.